BFP mRNA (Blue Fluorescent Protein mRNA) expresses blue fluorescent protein in cells, emitting light at approximately 443 nm. It’s used for visualizing cellular processes, gene expression analysis, protein localization, and multiplex imaging with other markers. As a transient expression system, BFP mRNA is ideal for experiments like live cell tracking and gene editing research.
Croyez’s BFP mRNAs was generated through in vitro transcription, and these mRNAs are then fortified at their 5' end by modified nucleotide capping, known as Cap1. To mimic the characteristics of fully processed mature mRNAs, we incorporate a poly(A) tail at the 3' end and optimize the mRNAs to enhance stability and overall performance. This ensures that the mRNAs function similarly to naturally occurring mature mRNAs in cells.